Enhanced resistance to <em>Listeria</em> infection in mice surviving sepsis: The role of lipid metabolism and myeloid cell reprogramming

This dataset contains raw and processed lipidomics data from an experiment investigating the lipid mechanisms of CD11b+ myeloid cells in mice surviving sepsis, through a murine cecal ligation and puncture (CLP) model. Single-cell RNA sequencing data of splenic myeloid cells are accessible in the GEO database (GSE249839). The data encompass untargeted plasma lipidomics, confocal microscopy of lipid droplets, quantitative RT-PCR, and survival and bacterial burden outcomes following Listeria monocytogenes infection. Lipid species were quantified using LC-MS/MS, and lipid class composition was analyzed. This dataset includes normalized lipid abundance tables, instrument settings, and group metadata. , Animal Model and Experimental Design Male C57BL/6J mice (8 weeks old) were used to model sepsis via cecal ligation and puncture (CLP), conducted under isoflurane anesthesia. Postoperative care included buprenorphine analgesia, subcutaneous resuscitation fluids, and one dose of imipenem/cilastatin. Sham controls underwent laparotomy without cecal manipulation. Mice were monitored daily, and moderate sepsis severity resulted in 20–30% mortality within 3–5 days. CLP survivors were studied at 4 and 12 weeks post-surgery. Listeria monocytogenes Infection and Bacterial Load Analysis At 12 weeks post-CLP or sham surgery, mice were intravenously challenged with 1×10⁷ CFU of Listeria monocytogenes (Lm). Survival was monitored for 7 days. For bacterial load quantification, mice were injected with 2×10⁶ CFU and sacrificed at 72 hours post-infection. Liver and spleen tissues were harvested, homogenized, serially diluted, plated on Brain Heart Infusion (BHI) agar, and incubated at 37 °C overnight. C..., # README: Enhanced resistance to listeria infection in mice surviving sepsis: The role of lipid metabolism and myeloid cell reprogramming **Dataset DOI: [10.5061/dryad.0p2ngf2cj](https://datadryad.org/submission/10.5061/dryad.0p2ngf2cj)** **Description of the data and file structure** This dataset contains raw and processed data supporting the manuscript: \"Enhanced resistance to *Listeria* infection in mice surviving sepsis: the role of lipid metabolism and myeloid cell reprogramming.\" EXPERIMENTAL GROUPS Group: Sham \- Surgical exposure without ligation or puncture. Group: CLP (Sepsis Survivors) \- Cecal ligation and puncture performed; samples taken 4–12 weeks post-surgery. **Files and variables** FILE: RE_Figure_1_Suvial_and_bacterial_load.csv **Description:** Survival (%) and CFU counts (log-transformed) in spleen and liver for sham and CLP mice 3 days post-infection. **Figure 1B**: Monocyte Analysis This section contains data on the percentage (%, left) and absolute co...,