Z. Xu, H. Ogawa, P. Vagnarelli, J.H. Bergmann, D.F. Hudson, S. Ruchard, T. Fukagawa, W.C. Earnshaw, K. Samejima (2011) CIL:12169, Gallus gallus gallus, memory B cell. CIL. Dataset

Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. INCENP localizes to centromeres in early mitosis, but fails to transfer to the spindle midzone during late mitosis in wild type clone 18 cells grown in the Aurora B inhibitor ZM447439. Cells were stained for INCENP (green), plus alpha-tubulin (red) and DAPI for DNA (blue). See Fig 6D panel 9 in Xu et al. 2009 J Cell Biol 187, 637-653.

DT40鸡B淋巴细胞系的细胞在有丝分裂阶段的图像。使用Deltavision系统上的100x/1.4NA计划阿波罗物镜捕获的宽场免疫荧光图像的Z系列，并使用约束迭代算法（Applied Precision）进行去卷积。展示的是中央Z切片。INCENP在早期有丝分裂中定位于着丝粒，但在野生型克隆18细胞中，在Aurora B抑制剂ZM447439处理的晚期有丝分裂期间，未能转移到纺锤体中区。细胞被染色以检测INCENP（绿色）、α-微管蛋白（红色）和DAPI（蓝色）用于DNA。请参考Xu等人2009年在《细胞生物学杂志》（J Cell Biol）发表的论文第187卷第637-653页的第6D部分第9图。