The frozen brain tissue (10 mg) was weighed into an Eppendorf tube and homogenized with 200 µL of methanol/water (v:v, 1:1). After thorough grinding, 200 µL of chloroform was added. The mixture was vo
C. elegans DAF-16/FOXO phosphorylation in different genetic backgrounds was analyzed by mass spectrometry of affinity purified HA-tagged DAF-16. Genetic backgrounds studied include par-1 hypomorphic m
Although persistent elevations in circulating glucose concentrations promote compensatory increases in pancreatic islet mass, unremitting insulin resistance causes a deterioration in beta cell functio
The liver is the central organ for carbohydrate metabolism to maintain the blood glucose concentration within a normal range. Glucose breakdown or synthesis in the liver is tightly regulated by insuli
