The presence of DNA methylation in beetles (Coleoptera) has only been investigated with bisulfite sequencing of Tribolium castaneum, which produced no evidence of DNA methylation. Here, we used whole
DNA methylation is an important epigenetic modification in eukaryotes. 5-methylcytosine (5-mC) is the most methylation modification in genomic DNA. In mammals, 5mC mainly presents in the promoters of