Calculated Maximal and EC50 Responses of AIS Mutations in HeLa Cells.

Values calculated by applying the 4-parameter curve fit algorithm available in SigmaPlot to multiple replicate experiments and represent Mean±S.E.M. All data are presented as absolute numerical measurements.Calculated maximal responses from AR HCA. EC50 values are presented for Nuclear Translocation, Hyperspeckling and Transcriptional Activity after stimulation with three AR agonists (DHT, Mibolerone and R1881) in HeLa transfected with wild type AR, AR-F764L, AR-R840C and AR-P766S. EC50 represents the nM concentration of agonist that provokes a response halfway between the baseline and maximal induction. Maximal response achieved with each agonist for each HTM parameter is expressed as % signal present in the nucleus for Nuclear Translocation, as fold-induction from time point 0 for Hyperspeckling and Transcriptional Activity.