Autofluorescent gut granules in L4/adults.

All strains were grown at 22°C. Individual L4 stage larvae/young adults were analyzed using fluorescence microscopy with a fluorescein isothiocyanate (FITC) filter and were scored for the number of autofluorescent organelles within the intestinal cells located between the pharynx and vulva. n = number of animals scored. 1glo-2(−)/glo-2(−) progeny of glo-2(−)/glo-2(+) hermaphrodite parents were scored. 2glo-2(−)/glo-2(−) hermaphrodites were mated with glo-2(+)/glo-2(+) males and the resulting glo-2(−)/+ animals were scored. 3hDf6/+ heterozygotes were generated by mating dpy-5(−)/+ males with unc-13(−) dpy-5(−) hDf6; hDp31 hermaphrodites and selecting Dpy non-Unc progeny, which were dpy-5(−)/unc-13(−) dpy-5(−) hDf6. These were allowed to self-cross. Homozygous hDf6 is recessive embryonic lethal [44] so 2/3 of the resulting progeny were predicted to be +/hDf6 and 1/3 were predicted to be +/+. 4glo-2(−)/hDf6 animals were generated by mating glo-2(−) dpy-5(−)/+ + males with unc-13(−) dpy-5(−) hDf6; hDp31 hermaphrodites and selecting Dpy non-Unc progeny, which were glo-2(−) dpy-5(−)/unc-13(−) dpy-5(−) hDf6. These were allowed to self-cross. Homozygous hDf6 is recessive embryonic lethal [44], so 2/3 of the resulting progeny were predicted to be glo-2(−)/hDf6 and 1/3 were predicted to be glo-2(−)/glo-2(−). The number of autofluorescent compartments in glo-2(zu455)/hDf6 was not significantly decreased compared to glo-2(zu455) χ2 P = 0.07. The number of autofluorescent compartments in glo-2(tm592)/hDf6 was decreased compared to glo-2(tm592) χ2 P = 0.001. 5Reciprocal crosses of glo-2(tm592) males with glo-2(zu455) hermaphrodites and glo-2(zu455) males with glo-2(tm592) hermaphrodites were performed to generate glo-2(tm592)/glo-2(zu455) adults. The L4 stage progeny of these animals were scored and were predicted to be ½ glo-2(tm592)/glo-2(zu455), ¼ glo-2(tm592)/glo-2(tm592), and ¼ glo-2(zu455)/glo-2(zu455).