In vivo timelapse imaging and analysis of Golgi satellite organelle distribution and movement in the neural progenitor cells of the brain

The dividing stem cells of the developing brain are the radial glial neural progenitor cells (NPCs), multifunctional cells that proliferate to generate all of the cells of the brain, but also act as scaffolds for their migrating neuron progeny, guideposts for pathfinding growing axons and regulators of synaptic activity. These remarkable cells perform these very different activities while remaining in contact with the inner and outer surface of the ever-growing brain. NPCs synthesize proteins locally to support the compartmentalized protein expression required for the cells to perform their specialized functions, but it is not clear how the necessary processing that normally occurs in the Golgi apparatus is achieved at locations far from the cell body. Golgi satellites, motile organelles, and members of the protein maturation machinery, control protein glycosylation and maturation in polarized cells like neurons. To investigate whether NPCs also rely on Golgi satellites, we expressed a ..., Confocal images acquired with Slidebook 2023 software (3i. Inc.) were exported as .tifs. , , # In vivo timelapse imaging and analysis of Golgi satellite organelle distribution and movement in the neural progenitor cells of the brain

[https://doi.org/10.5061/dryad.f1vhhmh3v](https://doi.org/10.5061/dryad.f1vhhmh3v)

3D timelapse confocal images and the files generated by FIJI/ImageJ to complete the morphometric analyses.

## Description of the data and file structure

Description of the Image Acquisition Equipment:

Confocal z-stacks of labeled cells ≤1 µm z-step intervals from the dorsal surface to ~150 µm depths using Slidebook image acquisition software (3i, Inc.) with the VIVO spinning disc confocal system composed of a Yokogawa CSU-X1 spinning disk confocal and a Photometrics Prime 95B sCMOS camera mounted on a Zeiss Axio Examiner microscope equipped with a C-APO 63x/1.15 objective. The 561 nm laser was used to excite the mCherry-GOLT3 and the 488 nm laser excited EGFP. Signals were distinguished with single bandpass Semrock 617/73 nm and 525/30 nm filters for the mCherry ...,