Investigating the Mechanism of Chinese Medicine Formula AACO Against Chronic Heart Failure by Network Pharmacology Analysis and Experimental Validation

A traditional Chinese medicine (TCM) formula, containing Astragalus membranaceus (Fisch.) Bunge, Aconitum wilsonii Stapf ex Veitch, Curcuma longa L., and Radix ophiopogonis (AACO), has clinically proven therapeutic value for the treatment of chronic heart failure (CHF). In this study, we explored the potential pharmacological mechanism underlying the activity of the AACO formula against CHF. All TAC mice were administered AACO solution at 0.386 µg/g (TCM group), or an equivalent amount of double-distilled water (control group), once daily for 8 weeks.Independent experiments were performed using different donors for each experiment.