Conditions for chloroplast markers from the Plant Working Group (www.kew.org/barcoding/protocols.html); conditions for psbA-trnH adopted from Kress et al. 2005.
Coleanthus subtilis (Tratt.) Seidel (Poaceae) is an ephemeral grass from the monotypic genus Coleanthus Seidl, which grows on wet muddy areas such as fishponds or reservoirs. As a rare species with st