Beta-naphthoflavone treated worms

The soil nematode Caenorhabditis elegans is one of the simplest animals having the status of a laboratory model. Its genome contains 80 cytochrome P450 genes (CYP). In order to study CYP gene expression in C. elegans mixed stages and synchronized hermaphrodites were exposed to 18 known xenobiotic cytochrome P450 inducers. Messenger RNA expression was detected by DNA arrays and semiquantitative RT-PCR. Using subfamily-specific primers, a pooled set of exon-rich CYP fragments could be amplified. In this way it was possible to systematically check the influence of different inducers on CYP expression at the same time. The well-known CYP1A inducers beta-naphthoflavone, PCB52, and lansoprazol were the most active and in particular they strongly induced almost all CYP35 isoforms. A few number of further CYP forms were found to be inducible by other xenobiotics like phenobarbital, atrazine, and clofibrate. In addition, a transgenic C. elegans line expressing GFP under control of the CYP35A2 promoter showed a strong induction of the fusion by beta-naphthoflavone in the intestine. Copyright 2001 Academic Press. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set秀丽隐杆线虫（Caenorhabditis elegans）是一类土壤线虫，同时也是最为经典的模式实验动物之一。其基因组中包含80个细胞色素P450（cytochrome P450，简称CYP）基因。为探究秀丽隐杆线虫体内CYP基因的表达特征，研究人员将混合发育阶段的线虫与同步化的雌雄同体个体暴露于18种已知的外源细胞色素P450诱导剂中。研究人员通过DNA微阵列（DNA arrays）与半定量逆转录聚合酶链式反应（semiquantitative RT-PCR）检测信使RNA的表达水平；借助亚家族特异性引物，可扩增得到一组富集外显子区域的CYP片段混合池。借此可同时系统性评估不同诱导剂对CYP基因表达的调控影响。已知的CYP1A诱导剂β-萘黄酮（beta-naphthoflavone）、多氯联苯52（PCB52）与兰索拉唑（lansoprazol）活性最强，尤其可强力诱导几乎所有CYP35家族同工型的表达。另有少量CYP亚型可被苯巴比妥（phenobarbital）、莠去津（atrazine）、氯贝丁酯（clofibrate）等其他外源化学物诱导。此外，在以CYP35A2启动子调控绿色荧光蛋白（GFP）表达的转基因秀丽隐杆线虫品系中，β-萘黄酮可在肠道内强力诱导该融合报告基因的表达。版权所有2001年学术出版社（Academic Press）。本数据集基于共享的生物学背景进行分组，涵盖物种、肿瘤类型、生物学过程等类别。关键词：逻辑集合（Logical Set）