TGF-ß specifies TFH versus TH17 cell fates in murine CD4+ T cells through c-Maf (Seq04, scRNA-seq)

Naive murine CD4+ T cells were cultured under different polarizing conditions in 96-well plates coated with anti-CD3/anti-CD28 for 0, 0.5, 1.5, 3.5 days, sorted by flow cytometry, and subjected to single-cell RNA-seq. Overall design: Seq04: scRNA-seq: Tfh(2)d0, Tfh(2)d0.5, Tfh(2)d1.5, Tfh(2)d3.5 MouseSampleTags Day MouseSampleTag01 0 MouseSampleTag02 0 MouseSampleTag03 0 MouseSampleTag04 0.5 MouseSampleTag05 0.5 MouseSampleTag06 0.5 MouseSampleTag07 1.5 MouseSampleTag08 1.5 MouseSampleTag09 1.5 MouseSampleTag10 3.5 MouseSampleTag11 3.5 MouseSampleTag12 3.5