Comparative analysis of gene expression in HepG2-NTCP_HA:HBc upon treatment with CAM-A or CAM-E

HepG2-NTCP_HA:HBc cells stably express HBV core antigen. Treatment of these cells with CAM-A compounds (but not CAM-E) induces the abnormal nuclear aggregation of core leading to cell death via apoptosis in a time dependent manner. The goal of this project is to determine the transcriptomic modifications induced by CAM-A treatment in these cell lines at day 2 and day 3 post-treatment. Overall design: HepG2-NTCP_HA:HBc cells were treated 24 hours post seeding with DMSO 2% (non treated control), CAM-Ah RG7907, CAM-At ALG5398 and CAM-E ALG26 (negative control) at 1 µM in culture medium in presence of 2% DMSO. Apoptosis activation in all conditions was mesured daily from day 1 to day 6 post-treatment. We observed activation of apoptosis only in CAM-A treated cells from day 2 post-treatment. Total RNA was extracted from cells at day 2 and 3 post-treatment send for RNAseq analysis.