Soluble plantain NSP increases the transmucosal short circuit current of ex vivo human ileal follicle-associated epithelium.

Plantain NSP (5 mg/mL) significantly increased (A) transmucosal short circuit current (Isc), with a concomitant decrease in (B) epithelial potential difference (PD, apical-side negative), during pre-treatment of, and blockade of translocation of S. Typhimurium across ex vivo human ileal follicle-associated epithelium (FAE) mounted in Ussing chambers. (C) Trans-epithelial electrical resistance (TEER) was maintained throughout the experiment. N = 4, with 2 tissue replicates in each case. * PPIsc and PD responses were calculated and expressed as the increment change (Δ) for each sampling period. Arrows at T0 min indicate addition of EGFP-expressing S. Typhimurium LT2 to the mucosal compartment (1×108 CFU/mL). Overnight culture of Ussing chamber serosal medium following 2 h infection had already demonstrated soluble plantain fibre to block translocation of Salmonella across isolated human FAE in this experiment; see reference [21].