Characterization of Arabidopsis thaliana MYB47 and MYB95 transcription factors in response to jasmonic acid in leaves.

We have found that Arabidopsis thaliana paralogous two MYBs, namely MYB47 and MYB95, regulate TSA1 and BGLU18 expression and ER-body formation in response to jasmonic acid (JA). To comprehensively understand the transcriptional regulation of these MYBs in response to JA, we conducted RNA-seq analysis between JA-treated rosette leaves of wild-type and myb47myb95 knockout mutants, together with myc2myc3myc4 mutant that is defective in master transcriptional regulator of JA signaling pathway. The transcriptome data analysis revealed that MYB47 and MYB95 regulate a subset of JA-responsive genes, including ER-body and defense-related genes. Overall design: RNA-seq of Arabidopsis leaves of wild type, myb47,95, and myc2,3,4 mutants that were treated with or without methyl jasmonate.