Differentially expressed stemness genes and regression coefficients obtained from survival analysis.

Here, positive log foldchange change (LFC) corresponds to upregulated genes and negative log foldchange corresponds to downregulated genes. LUAD cancer patient data is divided into high expression and low expression groups, with low gene expression used as reference values when performing Cox-proportional hazard model analysis [51] in Timer 2.0. The hazard ratio for each gene represents the impact of the corresponding gene expression change observed in high expression sample as compared to the low expression samples within the tumor samples. Genes with log foldchange greater than 2 or less than -2 across TCGA using DeSeq2 and GSE40419 datasets and GSE31210 dataset using Limma, and with hazard ratio greater than 1 with Benjamini and Hochberg (BH) adjusted p-value less than 0.05 is selected as key genes that are associated with loss of cancer patient survival. If gene differential expression test BH adjusted p-value is not smaller than 0.05, then it is represented with ns.