Summary of the properties of Eubacterium and Roseburia flagellin proteins and their predicted promoter and ribosome binding site sequences.

*Sequences were compared to the −35 and −10 recognition sequences for Butyrivibrio fibrisolvens σ28 and σ43, which are −35: TAAA (N16–17) −10: MCGATAa and −35: TTtACA (N19) −10: cATAAT respectively. The general bacterial consensus sequences for σ28 and σ43 are −35: TAAA (N15) −10: CCGATAT and −35: TTGACA (N15) −10: TATAAT respectively. † Predicted start positions were moved on the basis of alignment to amino-terminal sequences of E. rectale and R. inulinivorans flagellins. ‡ An alternative start codon exists three residues upstream of the predicted start position. Use of this alternative start codon would yield a distance of 8 bp between the predicted RBS and the start-codon.