Taxonomic annotation performance of Silva aligner for 16S rRNA sequences from Streptococcus and Rothia genomes.

Validation of Silva aligner (v1.2.11) taxonomic classification accuracy using 16S rRNA gene sequences extracted from genomes/MAGs used in this study. 16S rRNA sequences were extracted from S. salivarius (n = 3 genomes, 18 rRNA genes), R. mucilaginosa (n = 8 genomes, 10 rRNA genes), R. mucilaginosa_A (n = 2 genome, 3 rRNA genes), Streptococcus AIMSoral1 (n = 6 genomes, 8 rRNA genes) and Rothia AIMSoral2 (n = 2 genomes, 2 rRNA genes). Columns show: sequence_identifier (rRNA gene location in genome), genome annotation (GTDB-based species annotation), identity (% identity to Silva reference) and taxonomic assignments from five databases (lca_tax_embl_ebi_ena, lca_tax_gtdb, lca_tax_ltp, lca_tax_rdp, lca_tax_slv). (XLSX)