Urinary Extrecellular Vesicles miRNA Expression in Patients with Gitelman Syndrome: The Role of Hsa-let-7d-3p

Gitelman syndrome (GS) is a hereditary renal disorder caused by SLC12A3 mutations, resulting in salt-wasting and electrolyte imbalances. This study profiled miRNA expression in urinary extracellular vesicles (uEVs) and renal tissues from GS patients to explore the role of miRNA in renal tubular regulation. We aim to indetify the differnital expressed miRNA that may regulate renal transporters. Overall design: Urine samples were collected from 23 genetically confirmed GS patients and 23 healthy controls for uEV isolation, and renal biopsy tissues were obtained from 3 GS patients. Total RNA was extracted from uEVs ( uEVs combined into 4 GS groups vs 4 healthy control groups) and renal tissues, and small RNA sequencing was performed using the Illumina NextSeq 550 platform.