MEF2C knock-down in Human Skeletal Myoblasts. MEF2C knock-down in Human Skeletal Myoblasts

The main goal of this work was to determine the connection between MEF2C expression and its role in miRNA biogenesis in the differentiation of human skeletal muscle cells.In summary, our results demonstrated a significant role for MEF2C myogenic factor in sculpting the microtranscriptome during the differentiation of skeletal muscle cells. Overall design: miRNAs libraries from HSkM cell treated with control siRNA (n=3) and siRNA against MEF2C (n=3) were generated by deep sequencing, in triplicate, using the Illumina HiSeq 2000