本数据集围绕ITS1与ITS2作为真核生物DNA条形码的性能比较展开,包含大规模元分析所需的数据与代码文件。研究覆盖子囊菌、担子菌等十大类真核生物,涉及85,345个序列对,从PCR扩增、测序及物种鉴别三方面评估两者的效率与特性,为DNA条形码选择提供依据。 论文“ITS1: a DNA barcode better than ITS2 in eukaryotes?”
DNA barcode data hosted in the Data Portal of the Barcode of Life Data Systems. Records consist of specimen metadata, specimen images, and sequence data.
Supplemental Files Supplemental File 1. Species sampled in six dynamic plots for this study. Supplemental File 2. Alignment file of the three standard DNA barcode markers (rbcL, matK, and trnH
Diagnostic characters for each locus were identified with reference to GenBank sequence for the locus of particular species or related species (if not available in the GenBank data base). For ITS, the