Wolbachia endosymbionts manipulate the self-renewal and differentiation of germline stem cells to reinforce fertility of their fruit fly host

This data repository contains the data for the fertility plots, confocal images, and fluorescence plots in the manuscript titled "Wolbachia endosymbionts manipulate the self-renewal and differentiation of germline stem cells to reinforce fertility of their fruit fly host". There are tsv files for egg lay, egg hatch, and offspring counts, for all genotype and infection combinations reported in the paper, as indicated in the file names. These data are plotted in Figure 1 and 6 and supplementary figures S4, S8, S9. The raw Leica sample image files used to generate the micrographs and fluorescence intensity plots in Figures 1-6 and supplementary figures S5 and S7 are included (.lif files). There are also two tsv files for the fluorescence intensities from Bam and Sxl immunocytochemistry data points plotted in Figures 3 and 4 and supplementary figure S7. The R scripts written and used to plot these data and analyze their significance are also included. Leica .lif file naming (e.g., bam-vas-PI\_1\_\_mei-P26\_RNAi\_uninf-5d-210506.lif): The first gene names indicate the primary antibodies used and the counter stain, PI (e.g., bam-vas-PI, sxl-vas-PI). The Drosophila genotype is next (e.g., mei-P26\_RNAi, OreR, mei-P26\_1). Then, the infection state is listed (e.g., wMel or uninf(ected). The age of the flies dissected is listed next (e.g., 5d, 4-6d). Last, the date the sample was mounted and stored at -20 (and imaged within a month) is listed last. Note: Disregard the "*1*" notations - they do not distinguish these samples. Identity of secondary antibodies and fluorophores: Mouse primary antibodies were visualized with goat anti-mouse Alexa 405 secondary antibodies. Mouse antibodies were those against Hts, Bam, Sxl, and Orb. Rat primary antibodies were visualized with goat anti-rat Alexa 647 secondary antibodies. Rat antibodies were against Vas. Rabbit primary antibodies were visualized with goat anti-rabbit Alexa 405 secondary antibodies. Rabbit antibodies were those against pMad and pHH3.