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DNA damage-induced transcriptome changes in budding yeasts Saccharomyces cerevisiae and Candida glabrata

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https://www.ncbi.nlm.nih.gov/sra/SRP275841
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This study compared the transcriptional response to DNA damage in two closely related yeasts, S. cerevisiae and C. glabrata. Overall design: We cultured S. cerevisiae and C. glabrata in YPD containing 0.1% methyl methanesulfonate (MMS) for 1 hour, harvested the cells, isolated total RNA using the Qiagen Rneasy kit, and sent the RNA to Genewiz for RNAseq. Untreated cells were used as controls. 3 biological replicates for every condition were collected, except the S. cerevisiae no MMS condition, which had 2 biological replicates.
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2021-01-12
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