Transcriptional profiling sheds light on the cellular and fibrotic aspects of idiopathic subglottic tracheal stenosis

Transcriptional analysis of idiopathic subglottic tracheal stenosis Single cell RNA-sequencing gene expression comparison of subglottic tracheal stenosis with healthy trachea. Single-cell suspension from all samples were prepared using MACS Milteny Multi Tissue Dissociation Kit 1 (Miltenyi). Libraries were generated using Chromium Next GEM Single Cell 5´GEM, Library & Gel Bead Kit v5.2, Chromium Next GEM Chip K Single Cell Kit and Single Index Kit TT Set A (all 10x Genomics, Pleasanton, CA, USA).