Dataset for Astrocyte Mitochondria are a Sensitive Target of PCB52 and its Human-Relevant Metabolites

The C6 cells, a rat astroglial cell line was exposed to previously identified IC50 concentrations of PCB52 and its human-relevant metabolites, 4-OH-PCB52 and 4-OH-PCB52 sulfate. Various endpoint assays were performed to assess mitochondria as cellular targets of PCB52 and its human-relevant metabolites. The Glucose-galactose assay indicates that mitochondria may be the target of the three test compounds. Further, the DCFDA assay shows generation of oxidative stress and a fluorescence based assay for oxidative stress shows increased oxidative stress being generated in mitochondria of C6 cells exposed to PCB52 metabolites. JC-10 assay also shows loss of mitochondrial membrane potential, with 4-OH-PCB52 being the most potent compound. In addition, immunofluorescence assay for HSP60, a mitochondrial matrix protein, followed by confocal imaging show that mitochondrial morphology is altered distinctly by PCB52 and its human-relevant metabolites, 4-OH-PCB52 and 4-OH-PCB52 sulfate. Further, the seahorse assay assessing oxygen consumption rate in C6 cells is altered upon exposure to PCB52, 4-OH-PCB52, and 4-OH-PCB52 sulfate.