RNAseq of a unique human heart 5 days post-myocardial infarction versus healthy donor controls

Background: Myocardial infarction (MI) is a leading cause of death worldwide and can eliminate up to a third of the cardiomyocytes (CMs) within the human heart. Although CMs undergo mitosis during early development, most CMs cease cell cycling soon after birth. In contrast, rodent MI models have shown that CMs increase mitosis in response to ischemia, however this has not been shown in humans. Methods: Using a unique pre-mortem post-MI human heart, immunostaining, bulk RNA sequencing, proteomics, metabolomics, single nucleus RNA sequencing and a novel post-MI human biopsy method, we investigated human CM mitosis post-MI. Results: We show that adult human CMs exhibit increased mitosis and cytokinesis in response to ischemia. Conclusions: Future development of therapeutics to enhance this intrinsic mitotic potential could lead to new treatments that reverse heart failure via cardiac regeneration. This dataset is the bulk RNAseq dataset relating to a unique infarcted heart - left ventricle (6004 LV), right ventricle (6004 RV), right atrium (6004 RA) and healthy donor left ventricle (donor LV).