Transcriptome sequencing of Sour jujube

Investigating the molecular mechanisms of drought stress in "Shenmu" jujube seedlings. Transcriptome sequencing was performed on 15 uniformly growing seedlings. After full irrigation, drought stress was induced through natural water depletion. Three treatment groups were established: 0d (control), 14d (moderate), and 21d (severe), with five seedlings per group. Sampling occurred at 9:00 AM on each treatment day, collecting four apical leaves per plant. Three biological replicates were performed per group, yielding nine samples (3 g each).