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Differential mRNA expression upon microRNA-125a overexpression in murine hematopoietic cells

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE158126
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We report differential gene expression in hematopoietic cells (lineage-Sca+) upon overexpression and transplantation of murine hematopoietic stem cells. MicroRNA-125a was overexpressed in murine hematopoietic stem cells (LSK/SLAM) and transplanted into lethally irradiated reciepients. Twelve weeks after transplantation, 2000 (lineage-Sca+) bone marrow cells were directly sorted into lysis buffer and snap frozen immediately before moving to library prep.
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2021-02-12
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