Probe design for P224 FC and P30RC.

Probe P224FC and P30RC were designed for SNP P224 (C) and SNP P30 (G) as the extraction loci in forward (F) and reverse (R) orientation. (A) For different probe length (l), the G/C content, melting temperature (Tm), and ΔG value were calculated using Visual Omp. The success of separation by HSE with different probe lengths is given as the percent enrichment of one contributor with standard deviation and number of experiments (n). (−) indicates no data (B) Concentrations of hetero-duplex target probes (con) for match (M) and mismatch (MM) have been simulated for different probe lengths using Visual OMP with the assay parameters. Δ con presents the difference between match and mismatch concentrations.