ChIP of SMC2 at defined cell cycle stages

Investigating binding of the SMC2 protein genome-wide in synchronised HCT116 cells at early S, late S and G2/M Overall design: HCT116 cells were synchronised at the G1/S boundary and released either in normal media or in media supplemented with 0.4microM aphidicolin to induce replication stress. Cells were harvested at 4, 8 and 10h post release, corresponding to early S, late S and G2/M. Cells were fixed and ChIP for SMC2 was performed