Metabarcoding analysis of wheat crop plants under high basal nitrogen fertilization unveils that rizhosphere microbiomes are shaped by the plant and only particular bacterial and fungal taxons are affected by Trichoderma or top dressing application. (I) Bacterial community

Here, a long-term wheat field trial was performed to assess, under a high basal N fertilization, the influence of N top dressing and Trichoderma applications on the microbial assembly in the rhizosphere and also on crop yield. Neither top dressing nor T. harzinum T34 applications increased yield by themselves, but top dressing application significantly increased grain protein and gluten contents. We analyzed 16S rDNA in three different compartments (bulk soil, rhizosphere and root endosphere) at 12 days after top dressing and/or T. harzinum T34 application