Cycling stem cells regenerate the damaged intestinal epithelium upon irradiation

The intestinal epithelium undergoes DNA damage response, regenerative response and homeostasis after suffering irradiation. To understand the dynamic change of intestinal epithelium during the irrradiation recovery, we performed single cell RNA sequencing on irradiation-resistant +4 cells in homeostasis and its lineages at different time points after radiation. Overall design: We generated an Msi1CreERT2 allele for lineage tracing and observe that Msi1CreERT2-marked cells are enriched at the +4 crypt position which showed resistant to DNA damage. Msi1-CreERT2; R26-mTmG mice were administrated with 12Gy ?-IR after fifteen hours tamoxifen treatment. The epithelium were collected 0, 1, 2, 3, 5 days after irradiation, GFP cells were sorted and profiled by single cells RNA sequencing.