Quantification of colocalization of GAD67 with the postsynaptic marker PSD-95 and the presynaptic marker synaptophysin in spinal lamina II.

Using two different approaches for colocalization analyses (Mander’s colocalization coefficients and analyzing the relative area of combined channels), triple immunofluorescence staining of GAD67 with PSD-95 and synaptophysin revealed no significant difference in any of the parameters tested between sham-treated (n = 5) and CCI (n = 5) animals (two-way ANOVA, for all groups: P>0.05). Data: mean ± s.e.m.