241207_4DHSI_Bovine_Heart_LeftAtrium_RFA

Materials and Methods A fresh heart extracted from a male bovine (age: 1 year and 6-7 months, weight: 130-140 kg, death time: 11:15, heart removal time: 11:52) was delivered on ice to the Orbeli Institute, and dissection started at 13:04. Pieces of tissue were extracted from the left atrium. Three left atria tissue pieces were used for executing several RFA lesions and imaging. RFA machine (EPT-1000 XP, Boston Scientific) was set up at 8 W power and 15 s duration. The data with respect to each sample is shown in the following table. Ablation description: Sample 1 Starting time 13:15 Sample 2 Starting time 14:11 Sample 3 Starting time 15:14 Lesion number Duration (s) Lesion number Duration (s) Lesion number Duration (s) 1 15 1 15 1 15 2 15 2 15 2 15 3 15 3 15 3 (due to muscle contraction, catheter tip moved during RFA) 15 4 15 4 15 4 15 5 15 5 15 5 15 6 15 6 15 7 15 8 15 Hyperspectral Imaging (HSI) Images were taken under illumination with a light source constructed by the lab, consisting of a xenon lamp and filter wheel (343, 351, 360, 370, 380 and 390 nm). White light image was taken under illumination with white LED light. While imaging, the samples were immersed in cold PBS together with ice. Hyperspectral images were taken with a Nuance FX Imaging System (model: N-MSI-FX, CRi, Woburn, MA, USA). For each sample, several cubes were acquired under illumination with each of the wavelengths above mentioned, as described in the respective metadata files for each sample. Metadata_Sample1.csv Metadata_Sample2.csv Metadata_Sample3.csv Pixel Calibration Pixel size can be calculated based on the scale images acquired at approximately the same distance from the camera. These are available as: Scale_Sample1.tif Scale_Sample2.tif Scale_Sample3.tif In this dataset scale images are copies of the same for all samples, in order to facilitate programmatic access via parametrization. TTC After HSI acquisition, the ablation on the samples were revealed with 2,3,5-Triphenyltetrazolium chloride (TTC) staining. TTC solution was prepared the same day at 1% concentration. Two slices (denoted as “a” and “b”) were cut from the center of each lesion, and immersed in TTC solution. After at least 20 minutes of incubation, slices were photographed together with scale, for estimating lesion size. Respective data are available in the following folders: TTC_Sample1 TTC_Sample2 TTC_Sample3 Data Management This dataset has been organized and structured according to the following guidelines: https://doi.org/10.5281/zenodo.17176953