Transcriptional adaptation to chronic exposure to Cytolethal Distending Toxin in HeLa cells

The Cytolethal Distending Toxin (CDT) is a bacterial genotoxin that promotes persistent bacterial colonization during infection and is associated with many inflamatory disorders and cancer. At the cellular level, CDT induces DNA damage and inflammatory signatures in host cells. The aim of this study was to better understand the links betwen CDT-mediated genotoxic activity and immunomodulatory effects. HeLa cells were exposed to a chronic (8 weeks) treatment to 0.25 ng/ml of wild-type (WT) or the catalytic mutant H153A (HA) of CDT from Escherichia coli and transcription analysis was performed using Agilent Sureprint G3 Human GE v2 8x60k microarray. Human HeLa cells were exposed to 0.25 ng/ml of WT or HA CDT for 8 weeks,then RNAs were extracted.