Specific initiation by RNA polymerase I in a whole-cell extract from yeast.
收藏PubMed Central1991-02-01 更新2026-05-16 收录
下载链接：
https://pmc.ncbi.nlm.nih.gov/articles/PMC50943/
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资源简介：
A protocol is described for making a soluble whole-cell extract from yeast (Saccharomyces cerevisiae) that supports active and specific transcription initiation by RNA polymerases I, II, and III. Specific initiation by polymerase I decreases in high-density cultures, paralleling the decrease in abundance of the endogenous 35S rRNA precursor. This extract should be useful for studying the molecular mechanisms that regulate rRNA transcription in yeast. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1991-02-01
