Fate-resolved gene regulatory signatures of individual B lymphocytes in the early stages of Epstein-Barr Virus infection

Enriched human B lymphocyte sample were collected at key timepoints before and after infection with Epstein-Barr Virus (EBV) and analyzed to evaluate cellular heterogeneity with respect to gene expression and chromatin accessibility using single-cell multiomics library construction, sequencing, and analysis. These data were used to construct a multi-state model of post-infection dynamics and distinct B cell fate trajectories that arise from different cellular responses induced by EBV. These responses include antiviral interferon response induction, DNA damage responses and growth arrest, oncogene-induced senescence, B cell activation, and activation-induced differentiation into plasmablasts. Overall design: Primary B cells were enriched from PBMCs from two donors