Primers and amplicon characteristics for candidate internal control genes, AGO2 and RdR6.

List of N. benthamiana selected candidate reference genes: symbol, name, accession numbers, designed qRT–PCR primer sequences, length of the amplified fragment (A), melting temperature (Tm), efficiency of PCR amplification as calculated by CFX Manager Software (E), and the coefficient of determination (R2). Selected N. benthamiana candidate reference gene accessions are shown as identifiers of GenBank database, DFCI N. benthamiana Gene Index (TC) [91] and Sol Genomics Network (Niben) [92]. Their corresponding homologs in A. thaliana are also shown (in brackets).