five

Probabilistic modeling of ambient noise for multiple single-cell omics technologies

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NIAID Data Ecosystem2026-03-14 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP353567
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We conducted this CROP-seq study to evaluate the performance of scAR. We designed a viral pool of 99 sgRNAs targeting 13 different genes, most of them being essential in MCF7 cells. We infected MCF7 cells expressing dCas9-KRAB with the lentiviral libraries at extraordinary low multiplicity of infection (MOI=0.3) to ensure single integrations of sgRNAs. Cells were harvested at D5 or D12 post-transduction.
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2022-12-31
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