This study investigates the effects of controlled burning (black firebreaks) on soil prokaryotic community structure in a protected Brazilian savanna ecosystem. Soil samples were collected in the Parque Nacional da Chapada Diamantina (Bahia, Brazil) from burned and control areas, including superficial and subsurface soil layers. Prokaryotic communities (Bacteria and Archaea) were characterized using 16S rRNA gene amplicon sequencing targeting the V3–V4 region on the Illumina MiSeq platform (2×250 bp).

This study investigates the effects of controlled burning (black firebreaks) on soil prokaryotic community structure in a protected Brazilian savanna ecosystem. Soil samples were collected in the Parque Nacional da Chapada Diamantina (Bahia, Brazil) from burned and control areas, including superficial and subsurface soil layers. Prokaryotic communities (Bacteria and Archaea) were characterized using 16S rRNA gene amplicon sequencing targeting the V3–V4 region on the Illumina MiSeq platform (2×250 bp). The aim of this study is to evaluate how prescribed fire management influences microbial diversity and community composition in tropical soils.