Transcriptomic programs in the retina of a mouse model of hypertensive glaucoma modulated by retinal ganglion cell-targeted expression of SIRT1

We used bulk RNA sequencing to examine the molecular basis of adenoassociated virus (AAV)-SIRT1-based gene therapy in a model of ocular hypertension. Intraocular pressure (IOP) was elevated through intracameral injection of magnetic microbeads (MBs) for either 2 or 8 weeks. AAV-SIRT1 treatment is targeted to RGCs and prolonged for 4 weeks post-MB injection.