Data underlying publication "De novo fabrication of custom-sequence plasmids for the synthesis of long DNA constructs with extrahelical features"

Bulk and single-molecule raw data underlying the publication "<strong><em>De novo</em> fabrication of custom-sequence plasmids for the synthesis of long DNA constructs with extrahelical features</strong>", the research objective of which is the generation and validation of custom-sequence plasmids for the assembly of DNA constructs with extrahelical features for single-molecule experiments. The data include DNA gel images, as well as force-extension tables and fluorescence tracking data acquired using correlative dual-beam optical tweezers and confocal microscopy.

本数据集为发表论文《用于合成带有螺旋外特征的长DNA构建体的定制序列质粒的从头合成（De novo）》所依托的批量与单分子原始数据。该研究的核心目标为制备并验证定制序列质粒，以组装带有螺旋外特征的DNA构建体，用于单分子实验。数据集包含DNA凝胶电泳图像，以及通过关联式双光束光镊与共聚焦显微镜采集得到的力-伸长量数据表与荧光追踪数据。