Dataset for Detailed study of kinetics, stability, and fluorescent properties of cyclodextrin complexes for sensitive automated determination of diclofenac in the sequential injection system

Dataset covers all experiments in frames of the study of kinetics, stability, and fluorescent properties of cyclodextrin complexes for sensitive automated determination of diclofenac in the sequential injection system. Data were used to develop, optimise and apply the final method for determination of low levels of diclofenad in samples from metabolic study with primary human hepatocytes. Spectra in the file "Complexes of CD and DCF - offline" were measured using spectroffluorometer Shimadzu RF6000 with manual quartz cuvette (3 ml volume, 1 cm optical path), individual settings are included in figures. Measurements in flow system, based on sequential injection analysis (SIA) principle, were carried out using the same spectrofluorometer connected to the MicroSIA system (FIAlab), using the flow cell (100 microl volume, optical path 3x3 mm). Data records are available in two files corresponding to the type of cyclodextrin used - alfa and gama - "Complexes of CD and DCF-SIA system-alfa CD" and "Complexes of CD and DCF-SIA system-gama CD". In the last file measurements of real-life samples collected in metabolic study are included. All settings, control programs of the SIA system, timing, and concentrations of all solutions are given in figures.