Enhanced crosslinking and immunoprecipitation of SERBP1 in Hela cells

Purpose:We used eCLIP experiment to analyze binding profile of SERBP1 and its relationship with G4. Result: we found 505 high-confidence SERBP1 binding sites in Hela cells, and the analysis of sequence revealed transcriptome-wide enrichment of PQS in SERBP1-binding regions We first established a Hela cell line that stably expressed SERBP1, and then performed eCLIP experiments of SERBP1