Single-cell RNA sequencing analysis of left atrial appendage tissue following magnetogel-based occlusion in a porcine model

This study aims to evaluate the long-term biocompatibility and tissue integration of a novel magnetogel-based left atrial appendage (LAA) occluder. To assess the host tissue response, single-cell RNA sequencing (scRNA-seq) was performed on LAA tissue samples obtained from a preclinical porcine model after 10 months of magnetogel implantation. Tissue samples were collected from regions in direct contact with the magnetogel (contact zone) and adjacent non-contact areas. scRNA-seq analysis revealed the cellular composition, which included myofibroblasts, fibroblasts, neutrophil cells, macrophages, T cells, mast cells, smooth muscle cells, and Schwann cells. Critically, no significant differences in the ratios of immune cells (e.g., neutrophils, macrophages, T cells) or mast cells (indicative of allergic reactions) were observed between the contact and non-contact zones.