Induction of spontaneous human neocentromere formation and long-term maturation (QuantChIP-Seq)
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https://www.ncbi.nlm.nih.gov/sra/SRP276468
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资源简介：
We report a chromosome engineering system for human neocentromere formation and characterize the first experimentally generated human neocentromere. Neocentromere formation promotes local H3K9me3 eviction and cohesin and RNA polymerase II recruitment. Long-term culture results in gradual maturation of the inner centromere. Overall design: Examination of the indicated proteins occupancy before (parental cell line,S40-RPE) and after neocentromere formation (neocentromere cell line,Neo4p13) . Same analysis in Neo4p13 after long-term continuous culture (Neo4p13_100d and 200d). All samples were spiked-in 1% crosslinked immortalized mouse embrionic fibroblats (MEFs) for quantitative analysis.
创建时间：
2021-01-19
