Directed-evolution of DENV2 prM cleavage site in Vero and C6/36 cells
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA719861
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A saturation mutagenesis library was created at the DENV2 prM furin cleavage site sequence, and reverse genetics used to create a viral library. The parental viral library was passaged three times in either Vero 81 (African Green Monkey) or C6/36 (Aedes albopictus) cells. Targeted high-throughput sequencing was performed to identify changes at the furin cleavage site
创建时间:
2021-04-05



