The role of RUNX2 and BHLHE40 in pathologically relevant CD4-positive tissue-resident T-cells in Crohn's disease. (scMultiome)

Tissue-resident T-cells (TRM) are deeply involved in immune memory at the site of inflammation. Here, we identified two key transcription factors, RUNX2 and BHLHE40 as regulators of pathologically relevant CD4-positive TRM in the inflamed gut mucosa of Crohn’s disease patients. CD4-positive T cells were sorted from mononuclear cells derived from surgically resected gut specimens by flow cytometry. Nucei were extracted from CD4-positive T cells and subjected to scMultiome analysis.