CD8+ T-cell plasticity regulates vascular regeneration in type-2 diabetes. Mus musculus

The subsets of CD8+ T-cells regulating tissue repair and regeneration have not been studied. To delineate the heterogeneity of CD8+ T-cells in non-lymphoid tissues of Lepr db/+ and Lepr db/db mice, we performed large-scale, droplet-based single-cell transcriptomic profiling. We purified about ~ 1,000 CD45+CD3+CD8+ T-cells from the ischemic muscles of Lepr db/+ and Lepr db/db, respectively, at day 7 after injury. After filtering, we obtained 934 and 768 cells from Lepr db/+ and Lepr db/db, respectively; and performed unsupervised analysis.