RNA sequencing of murine C2C12 cell treated with high-throughput screening hit compound 6,8-dimethyl-3-(4-phenyl-1H-imidazol-5-yl)quinolin-2(1H)-one.

The hit compound promotes expression and acitivity of alkaline phosphatase in murine cells that are bipotential for myogenic and osteoblastogenic differentiaion. RNA sequecing was performed to assess global transcript expression, with particular attention to genes indicative of osteoblast and bone differentiation. For RNA-Sequencing total RNA was isolated with Trizol reagent (Invitrogen 15596026) from murine C2C12 cells cultured for two days in DMSO (1% in culture medium) or 10 μM 6,8-dimethyl-3-(4-phenyl-1H-imidazol-5-yl)quinolin-2(1H)-one (n=3 each).