Culture Condition of Bone Marrow Stromal Cells Affects Quantity and Quality of the Extracellular Vesicles

Extracellular vehicles (EVs) released from bone marrow stromal cells (BMSCs) have been demonstrated to play an important role in their therapeutic actions for various diseases. This study establishes a scalable and reproducible EV manufacturing processes needed to develop successful implementation of EV therapy for patients. Using human BMSCs isolated from nine healthy donors, researchers examined the effects of high-performance culture media that can rapidly expand BMSCs on EV production and quality in comparison with the conventional culture medium.

骨髓基质细胞（BMSCs）释放的细胞外囊泡（EVs）已被证实在其治疗多种疾病中的作用中扮演着至关重要的角色。本研究确立了可扩展且可复制的EV制造工艺，这对于成功实施EV疗法至关重要。研究人员利用从九位健康捐赠者中分离出的人BMSCs，研究了高性能培养基对BMSCs快速扩增以及EV生产与质量的影响，并与传统培养基进行了比较。